Thioredoxin, Recombinant, Yeast

Thioredoxins are a group of ~12kD redox proteins that mediate numerous cytosolic processes in all cells. Human thioredoxin can be exported out of the cell where it has additional functions including the ability to stimulate cell growth. Recombinant yeast Thioredoxin is a single, non-glycosylated, polypeptide chain having a molecular mass of 12.6kD. , Dimers and Aggregates:, < 1% as determined by silver-stained SDS-PAGE gel analysis. Biological Activity: The specific activity was found to be ~3IU/mg. Unit Definition: One unit equals the change of A=1 unit in absorbance at 650 nm at 25°C using 0.13uM bovine insulin containing 0.33mM DTT (pH 6.5). Endotoxin: < 0.1ng/ug (IEU/ug) of Thioredoxin Reductase, Reconstitution: Reconstitute the lyophilized Thioredoxin in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.