Taste Receptor 1, Rat Control Peptide (TR1)

TR1 (rat 840 aa) and TR2 (rat 843 aa) share ~40% homology with each other, and ~30% with CaSR, and 22-30% with V2R pheromone receptors and mGLURs. Rat TR are 7 TM domain containing protein with an extra long N-terminal, extracellular domain (1). A 24aa Peptide sequence near the C-terminus of rat TR1. Specific for rat TR1. No significant sequence homology with TR2 or gustducin or pheromone receptors. Higher vertebrates are believed to possess at least five basic tastes: Sweet, bitter, sour, salty, and unami (the taste of monosodium glutamate). Taste receptor cells that may selectively reside in various parts of the tongue and respond to different tastants and perceive these taste modalities. Circumvallate papillae, found at the very back of the tongue, are particularly sensitive to biter substances. Foliate papillae, found at the posterior lateral edge of the tongue, are sensitive to sour and bitter. Fungiform papillae at the front of the tongue specialize in sweet taste. , Recently, two novel taste receptors, TR1 and TR2, have been cloned with distinct topographical distribution in taste receptor cells and taste buds. TRs are members of a new group of 7 TM domain containing GPCR distantly related to other chemosensory receptors (Ca+-sensing receptor (CaSR, a family of putative hormone receptor (V2R), and metabotropic glutamate receptors). TR1 is expressed in all fungiform taste buds, whereas TR2 localized to the circumvallate taste buds. Both receptors do not colocalize with gustducin. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.