SARS-CoV-2 Mpro Protein

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped positive-stranded RNA virus and the causative agent of COVID-19, a primarily respiratory illness characterized by fever, cough, and shortness of breath that can lead to life-threatening complications. The SARS-CoV-2 genome contains approximately 30 kilobases and 14 open reading frames (ORFs) that encode four structural proteins: spike, envelope, membrane, and nucleocapsid, as well as 16 non-structural proteins and 9 accessory factors. The SARS-CoV-2 main protease (Mpro), also known as 3C-like protease (3CLpro), is encoded within the non-structural protein 5 (nsp5) region of ORF1ab. Auto-activation of SARS-CoV-2 Mpro cleaves nsp5-16, which, together with the nsp1-4, form the SARS-CoV-2 replication and transcription complex (RTC), which is critical for the viral replication cycle. Inhibition of SARS-CoV-2 Mpro activity reduces SARS-CoV-2 replication in infected cells in vitro, as well as decreases lung viral loads and pulmonary lesions in ACE2 transgenic mice infected with SARS-CoV-2. Cayman's SARS-CoV-2 Mpro protein can be used for enzyme activity assays.Synonyms: 3C-like Proteinase, Coronavirus Endopeptidase C30, Peptidase C30, Severe Acute Respiratory Syndrome Coronavirus 2 Mpro. Purity: >95% estimated by SDS-PAGE. Source: Active recombinant SARS-CoV-2 Mpro expressed in E. coli. Amino Acids: 1-306, Location on P0DTD1 3,264-3,569. MW: 33.9 kDa. Formulation: (Request formulation change), 20 mM HEPES, pH 7.5, with 2.5 mM DTT and 10% glycerol. UniProt Accession #: P0DTD1.