Rift Valley Fever Virus L Protein (TAN/Dod-002/07) (recombinant)

Rift Valley fever virus (RVFV) is a single-stranded negative-sense RNA virus, member of the Phlebovirus genus, and mosquito-transmitted pathogen endemic to sub-Saharan Africa and the Arabian peninsula. The tripartite RNA genome of RVFV is composed of three segments: L, which encodes L protein, a single-polypeptide RNA-dependent RNA polymerase (RdRp), M, which encodes a single open reading frame (ORF) that produces the envelope glycoproteins Gn and Gc and non-structural proteins NSm and Gn/NSm fusion protein, and S, which is ambisense, encoding the nucleoprotein N in the genomic sense orientation, and the non-structural protein and major virulence factor NSs in the antigenomic orientation. RVFV L protein is composed of an N-terminal endonuclease domain linked to a PA-C-like domain, an RdRp core, a PB2-N-like domain, a CBD domain, and a C-terminal lariat domain. The N-terminal endonuclease domain is essential for cap-snatching during host mRNA transcription, and the RdRp core is essential for viral RNA production. Expression of a dominant-negative L protein mutant inhibits viral gene expression in RVFV-infected BHK/T7-9 cells. Cayman's Rift Valley Fever Virus L protein (TAN/Dod-002/07) (recombinant) consists of 256 amino acids and has a calculated molecular weight of 29.4 kDa.Synonyms: RVFV RNA-directed RNA Polymerase L. Purity: >90% as estimated by SDS-PAGE. Source: Recombinant RVFV C-terminal His-tagged L protein expressed in E. coli. Amino Acids: 1-250. MW: 29.4 kDa. Formulation: (Request formulation change), Lyophilized from sterile PBS, pH 7.4. UniProt Accession #: F4ZDJ3.