Recombinant Human Receptor Activity-Modifying Protein 1

Source : Escherichia Coli. RAMP1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 114 amino acids (27-117) and having a molecular mass of 12.9 kDa. RAMP1 is fused to a 23 amino acid His-tag at N-terminus. RAMP belongs to the type I transmembrane family of proteins called RAMP (receptor/calcitonin activity modifying proteins) which include an extracellular N terminus and a cytoplasmic C terminus. RAMPs are essential for CRLR transport to the plasma membrane. CRLR (calcitonin-receptor-like receptor) has seven transmembrane domains. Depending on which members of the RAMP family are expressed CRLR receptor functions as either an adrenomedullin receptor or a calcitonin-gene-related peptide (CGRP) receptor. In the presence of RAMP1, CRLR functions as a CGRP receptor. The RAMP1 protein takes part in the terminal glycosylation, maturation, and presentation of the CGRP receptor to the cell surface.