Protein Kinase A, Holoenzyme Type 2a, Recombinant (PKA)

Inactive human holoenzyme consists of one dimeric regulatory subunit type II alpha and two monomeric catalytic subunits (cAMP-free). Dimers and Aggregates: < 1% as determined by silver-stained SDS-PAGE gel analysis. Biological Activity: Recombinant Holo enzyme can be activated by adding the second messenger cAMP (Activation constant about 100nM) releasing two monomeric catalytic subunits. Applications: The product is suitable for analysing PKA type II agonists (cAMP analogs) or antagonists. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.