Phosphatase, Alkaline, Chicken Intestine (EC 3.1.3.1)

Alkaline phosphatase is a broad term associated with non-specific phosphomonesterases with activity optima at alkaline pH. Activity: >0.9 units per mg dry weight ( 25ºC, pH 8.8), Unit Definition: 1 unit hydrolyzes 1umole of o-carboxyphenyl phosphate per minute at 25ºC, pH 8.8. Composition: The enzyme is a zinc metallo-enzyme. Schüssler (1968) reports four isozymes. Chang and Moog (1972) found three isozymes in the enzyme from chicken duodenum (This preparation is from the whole intestine.), Optimum pH: 8-9, A280@mg/ml: As Reported, Activators: Schüssler (1968) indicates activation by Mg2+. See Sivanaesan et al. (1991). Inhibitors: Acidification to pH 4.5 reversibly inactivates the enzyme. Quality Control: SDS-PAGE, , Storage and Stability:, Lyophilized powder may be stored at 4°C. Stable for 6 months after receipt at 4°C.