Peroxidase, Horseradish (HRP)

HRP is widely used as an enzymatic label in immunoassays. Usually, the enzyme is coupled to antibodies, lectins or haptens. Coupling to antibodies etc. may be performed through the carbohydrate side chains of the HRP. HRP consists of the basic isoenzyme having a molecular weight of 44kD. Activity: > 250 U/mg (25°C, guaiacol as the hydroogen donor, pH 7.0 and H2O2 as substrate). Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.