Pectate lyase 1B, Erwinia chrysanthemi

Pectate lyase 1B (EcPel1B), assigned the E.C. number 4.2.2.2, is a derivative of Erwinia chrysanthemi. It is an endo-1,4-alpha-polygalacturonic acid lyase. The recombinant EcPel1B, purified from Escherichia coli, is a single-domain Pectate Lyase family 1 (PL1) enzyme (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 1 mg/mL. Bulk quantities of this product can be made available upon request. Shipping Conditions: Shipping: Dry Ice to RT. Storage Conditions: -30°C to -15°C. Class: Polysaccharide Lyase. Activity: Pectate lyases. Family: PL1. Ec Number: 4.2.2.2. Organism: Erwinia chrysanthemi. Architecture: PL1. Optimal pH: 9. Optimal Temperature: 50°C. Substrates: polygalacturonic acid (PGA)