Oligoalginate lyase 15A, Sphingomonas sp.

Oligoalginate lyase 15A (SAlg15A), assigned the E.C. number 4.2.2.26, is a derivative of Sphingomonas sp. It is an enzyme that catalysis the degradation of alginate oligosaccharides by a beta-elimination mechanism. The recombinant SAlg15A, purified from Escherichia coli, is a single-domain Pectate Lyase family 15 (PL15) enzyme (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 0,5 mg/mL. Bulk quantities of this product can be made available upon request. Shipping Conditions: Shipping: Dry Ice to RT. Storage Conditions: -30°C to -15°C. Class: Polysaccharide Lyase. Activity: Alginate lyases. Family: PL15. Ec Number: 4.2.2.26. Organism: Sphingomonas sp. Architecture: PL15. Optimal pH: 8.5. Optimal Temperature: 37°C. Substrates: unsaturated oligoalginates