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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
N6000-35H.50 | 50 µg | - | - |
3 - 19 business days* |
947.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
NTB-A (NK-T-B-antigen), also known as Ly108 and SLAMF6, is a 60kD type I transmembrane... more
Product information "NTB-A, Fc Chimera, Recombinant, Human (SLAMF6)"
NTB-A (NK-T-B-antigen), also known as Ly108 and SLAMF6, is a 60kD type I transmembrane glycoprotein that belongs to the SLAM subgroup of the CD2 family (1). Mature human NTB-A contains a 205 amino acid (aa) extracellular domain (ECD) with one Ig-like V-set and one Ig-like C2-set domain. It also contains a 21 aa transmembrane segment and an 84 aa cytoplasmic domain with two immunoreceptor tyrosine-based switch motifs (ITSMs) (2, 3). An alternately spliced isoform is truncated in the cytoplasmic domain and lacks the two ITSMs. Within the ECD, human NTB-A shares 48% aa sequence identity with mouse and rat NTB-A. The ECD of human NTB-A shares 19%-34% aa sequence identity with comparable regions of human 2B4, BLAME, CD2F-10, CD84, CD229, CRACC, and SLAM. NTB-A is expressed on the surface of NK, T, and B lymphocytes as well as eosinophils (2, 4, 5). It interacts homophilically through weak associations between the Ig-V domains (2, 5-7). NTB-A functions as an activating coreceptor on NK and T cells (2, 5, 6, 8). Tyrosine phosphorylation in the membrane proximal ITSM enables specific association with EAT-2, an interaction that is required for NTB-A mediated cytotoxicity of NK cells (9). Phosphorylation-dependent NTB-A association with SAP is required for full production of IFN-gamma by NK cells (5, 9). This interaction is independent of EAT-2 binding and appears to involve the membrane distal ITSM (5, 9). NTB-A deficient mice show weakened Th2 responses and elevated levels of neutrophil-derived inflammatory mediators (10). On B cells, NTB-A modulates immunoglobulin class switching and the balance between tolerance and autoimmunity (5, 11). Source: DNA sequence encoding the extracellular domain of human NTB-A (Leu 28-Lys 225, Accession # Q96DU3) was fused to the Fc region of human IgG1 via a peptide linker. The chimeric protein was expressed in a mouse myeloma cell line, NS0. , Definition: Measured by its ability to bind biotinylated rhNTB-A in a functional ELISA type binding assay. Applications: Suitable for use inWestern Blot. Other applications not tested. Recommended Dilution: Western Blot: 48.9kD migrates as an ~ 65-80kD protein in SDS-PAGE under reducing conditions. , Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Keywords: | KALI, CD352, NK-T-B-antigen, SLAM family member 6, Activating NK receptor |
Supplier: | United States Biological |
Supplier-Nr: | N6000-35H |
Properties
Application: | WB |
Conjugate: | No |
Species reactivity: | human |
Format: | Highly Purified |
Database Information
KEGG ID : | K16852 | Matching products |
UniProt ID : | Q96DU3 | Matching products |
Gene ID | GeneID 114836 | Matching products |
Handling & Safety
Storage: | -20°C |
Shipping: | +4°C (International: +4°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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