NaDC-1, Mouse, Control Peptide (Sodium Dicarboxylate Transporter)

The sodium-dicarboxylate cotransporters (Solute carrier family 13) , which transport succinate and other Krebs cycle intermediates, fall into 2 categories based on their substrate affinity, low affinity and high affinity. Both the low- and high-affinity transporters play an important role in the handling of citrate by the kidneys. The two different Na+-coupled dicarboxylate transporters (NADC) have been identified in mammalian tissues. These are NADC1and NADC3 (NADC2 is found in C. elegans). NADC1 is Na+-coupled, electrogenic, and exhibits low affinity for its dicarboxylatesubstrates. This isoform is expressedprimarily in the brush border membrane of intestinal and renalepithelial cells. The physiological function of NADC1 is to absorbthe intermediates of the citric acid cycle, such as citrate, succinate,-ketoglutarate, fumarate, and malate, in the intestine and kidney.NADC3 is also a Na+-coupled and electrogenic dicarboxylate transporter, but it exhibitsrelatively higher affinity for its substrates compared with NADC1. The NADC3is expressed primarily in the basolateral membrane of intestinaland renal epithelial cells. However, it is also found in tissuessuch as liver, placenta, and brain. NADC3 in the kidney is involvedin generating the driving force for the organic anion transporterOAT1 to facilitate the active entry of organic anions into thetubular cells across the basolateral membrane. In the brain,NADC3 mediates the cellular uptake of N-acetylaspartate, a processclosely linked to myelination. Therefore, the physiologicalfunctions of the NADCs may extend beyond the mediation of cellularentry of citric acid cycle intermediates. Recently, a third member of this familyin mammals has been identified, Na+-coupled citrate transporter (NACT), mediates the cellular uptakeof citrate in a Na+-coupled manner. , NADC-1, (SLC13A2 gene) is involved in a sodium dicarboxylate cotransport, an electrogenic process, coupling 3 sodium ions to the transport of each divalent anion substrate. NADC-1 is a 586aa protein in mouse, 587aa in rat and 592aa in human (chr 17p11.1-q11.1). In vitro translation of human NADC-1 produced an approximately 48kD protein. The deduced aa sequence of human NADC-1 is 78% identical to rabbit NADC-1 and 47% to rat sodium sulfate transporter. NADC-1 transporter is a low affinity, sodium dependent, and pH-insensitive transporter of succinate, its transport of citrate is stimulated by acidic pH. Source: 15aa peptide of mouse NADC-1, Location: ~C-terminal, Species Sequence Homology: Rat-92%. Applications: Suitable for use in ELISA as a control peptide for N0015B. Other applications not tested. Recommended Dilution: ELISA: Coat ELISA plates at 1ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.