MLL1 (human recombinant)

Mixed Lineage Leukemia (MLL1) plays a major role in epigenetic regulation through methylation of histone 3 at lysine 4 (H3K4) to activate gene transcription. Methylation of H3K4 leads to upregulation of developmental genes, including HOX family members. In addition to its methylation activity, MLL1 has been shown to co-localize with Pol II at several genomic elements. Consequently, MLL1 has very low basal methyltransferase activity unless complexed with the activating protein complex of WDR5, Ash2L, and RbBP5 (MLL/WAR complex). This C-terminal fragment of MLL1, residues 3762 - 3969, contains the SET1 domain for methyltransferase activity, and the WIN motif for binding the WAR complex. The protein DPY-30 has also been reported to associate with the MLL1-WAR complex. MLL1 is also available as a component of the MLL1/WAR complex (Cay-10756) and MLL1/WARD complex.Synonyms: ALL1, CXXC-type zinc finger protein 7, HRX, KMT2a, Lysine Methyltransferase 2A, Mixed Lineage Leukemia 1. Purity: >90% estimated by SDS-PAGE. Source: Recombinant protein expressed in E. coli. Amino Acids: 3,762-3,969 (N-terminal truncation). MW: 24.1 kDa. Formulation: (Request formulation change), 50 mM Tris, pH 8.0, with 400 nM sodium chloride, 10% glycerol, and 5 mM 2-mercaptoethanol. License: SUMOpro tag was used under non-exclusive license from LifeSensors, Inc.. UniProt Accession #: Q03164.