MBD2 (human recombinant, methyl binding domain aa 150-220)

DNA methylation occurs mainly at the 5'-position of cytosine rings (5-methylcytosine (5mC)). The 5mC bases are found largely in genomic regions with a high frequency of cytosines and guanines, called CpG islands. Methyl-CpG binding domain 2 (MBD2) protein specifically binds to methylated promoters on CpG islands. The MBD protein family consists of five members: MeCP2, MBD1, MBD2, MBD3, and MBD4. The sequence similarity between the MBD proteins is limited to the MBD domains. MBD2 binding to 5mC facilitates the recruitment of chromatin remodeling and transcriptional repressor complexes, which results in a repressive chromatin state. MBD2 has been implicated in breast cancer progression. Silencing of MBD2 protein expression was found to be sufficient to initiate and maintain tumor suppressor gene transcription.Synonyms: Methyl-CpG Binding Domain Protein 2, Methyl Cytosine Binding Domain Protein 2. Purity: >95%. Source: Recombinant N-terminal GST-tagged protein expressed in E. coli. Amino Acids: 150-220 (partial protein). MW: 34.8 kDa. Formulation: (Request formulation change), 50 mM Tris, pH 7.5, with 500 mM sodium chloride, 5% glycerol, and 5 mM beta-mercaptoethanol. UniProt Accession #: Q9UBB5.