Leptin, Recombinant, Rat (Obese Protein), Highly Purified

Leptin (Anti-Obesity Protein) is the protein product of the ob (obese) gene in mice. It seems to be involved in appetite control. The ob gene is expressed in adipose tissue and is thought to regulate the body's fat stores. Mice with the ob/ob genotype also develop a form of diabetes similar to type II (non-insulin dependent) diabetes. Recombinant Rat Leptin is a single, non-glycosylated, polypeptide chain containing 147 amino acid residues with a molecular weight of 16.2kD. , AA Sequence: MVPIHKVQDD TKTLIKTIVT RINDISHTQS VSARQRVTGL DFIPGLHPIL SLSKMDQTLA VYQQILTSLP SQNVLQIAHD LENLRDLLHL LAFSKSCSLP QTRGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDLSPEC, , Biological Activity: Determined by in vivo assay using the ob/ob mouse obesity model and NZO. Both strains of mice were treated via intraperitoneal injection once daily at a dosage of 5ug Leptin (L1670-16)/g of body weight for 7 days. Significant effects on body weight, food consumption and plasma glucose levels were observed compared to saline-treated controls. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O with 0.1% HSA or BSA and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.