Integrin alpha 11 beta 1, CF, Recombinant, Mouse, aa23-1141 (Integrin alpha11beta1)

Integrin alpha11beta1 is a noncovalent heterodimeric collagen receptor that contains a 150kD alpha11 subunit complexed to a 130kD beta1/CD29 subunit. alpha11beta1 is the only known alpha11-containing integrin. By contrast, at least twelve alpha-integrins share the beta1 subunit (1-5). alpha11beta1 is expressed on fibroblasts from cornea, skin and periodontal ligament (3-9). It is also expressed on mesenchymal stem cells and is considered a marker for non­muscle mesenchymal tissue (3, 8­10). Expression of alpha11beta1 is stimulated by TGF­beta1, activin A, and type I interferons, and down­regulated by FGF­2 (6, 9-11). The alpha11 extracellular domain (ECD) contains a vWFA domain which includes the ligand binding site (2­5). The beta1 ECD also contains a vWFA domain, which participates in binding. Each subunit has a transmembrane sequence and a short cytoplasmic tail. The dimer assumes a folded configuration at "rest". Divalent cations and intracellular (inside-out) signaling convert it to an active, extended and open conformation (1, 2). The 1119 amino acid (aa) mouse alpha11 ECD shares 90% and 95% aa sequence identity with rat and human alpha11, respectively, while the 708aa mouse beta1 ECD shares 98% aa identity with rat and 93­94% aa identity with human, bovine, porcine, ovine, canine and feline beta1. I domain­containing beta1 integrins alpha1beta1, alpha2beta1, alpha10beta1 and alpha11beta1 all bind collagens (3-5). alpha11beta1 preferentially binds interstitial collagens such as type I (5, 8, 12). It can contract collagen matrices and influence inflammatory edema (3, 13). Depending on context, alpha11beta1 can support or inhibit cell migration on collagen (7, 8). It is frequently up-regulated in the stroma of lung adenocarcinomas and enhances tumorigenicity (14, 15). alpha11beta1 is also involved in myofibroblast differentiation in human corneal fibroblasts and human cardiac fibroblasts (9, 16). Source: Recombinant protein corresponding to aa23-1141 from mouse Integrin alpha 11 beta 1, CHO-derived. Molecular Weight: ~115-135kD, Endotoxin: <0.10 EU/1ug (LAL method), Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute in sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.