Histone Deacetylase, Assay Developer (2X) (HDAC)

HDAC assay developer is an essential reagent for signal generation in HDAC or sirtuin assays done with any of HDAC fluorometric substrates. An appropriate fluorometric substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the HDAC assay developer produces a fluorophore. Typically, an 'inhibitor stop' will be included in the HDAC developer (e.g.Trichostatin A for class I and II HDACs, nicotinamide for sirtuins). Applications: Study of HDAC or sirtuin kinetics, regulation and inhibitor sensitivity. Ideal for drug discovery and HTS applications. Cell-based HDAC assays. Storage and Stability: -70°C. Store, long term, only as the 2X concentrate, at -70°C. Concentrate is stable for at least 3 freeze-thaw cycles, but care should be taken that both freezing and thawing steps are done quickly (e.g. liquid N2 or dry ice/EtOH for freezing, rapid finger-rubbing for thawing). Frozen storage of smaller aliquots is recommended.