Hemoglobin A1C, Highly Purified

Hemoglobin is composed of one globin plus four hemes. Heme consists of protoporphyrin IX and ferrous iron. The iron content of human hemoglobin is 0.338% (w/w) which gives a minimal molecular weight of 16,520. Ultracentrifugal and osmotic pressure measurements indicate an actual molecular weight four times the minimum molecular weight. Contaminants: No reaction against any glycohemoglobins, including HbA1a, HbA1b, HbF and HbAo. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.