Heat Shock Protein 70B_, Recombinant, Human (HSP70B')

In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. One class of stress proteins is the Hsp70 family which is comprised of a group of highly conserved, ubiquitous proteins of ~70kD that are located within different intracellular compartments and involved in a variety of cellular processes such as protein trafficking, folding, and prevention of aggregation. One strictly inducible member of the Hsp70 family is Hsp70B'. In humans, the hsp70B' gene is 77% similar to hsp70 and it encodes a ~70kD protein which is more basic than Hsp70 (1). Hsp70 and Hsp70B' differ in their extent and pattern of inducibility in response to conditions such as heat shock and CdCl2 treatment (1). In humans, Hsp70 is constitutively expressed under normal conditions, but Hsp70B' is only induced in response to stress (1). There is no basal expression of Hsp70B' (1). Applications: Suitable for use as a positive control for Hsp70B' antibody in Western Blots. On a 15-well minigel system, 100ng of lysate should be sufficient when used in a colorimetric Western Blot with Hsp70B' antibodies. Other applications not tested. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.