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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
351270.100 | 100 µg | - | - |
3 - 19 business days* |
719.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
Glutathione S-transferase mu 5, also known as GSTM5, is member of the glutathione s-transferase... more
Product information "GSTM5, Recombinant, Human, aa1-218, His-Tag (Glutathione S-transferase Mu 5, GSTM5-5, GTM5)"
Glutathione S-transferase mu 5, also known as GSTM5, is member of the glutathione s-transferase (GST) family of proteins. There are eight families of GST proteins, namely alpha, kappa, mu, omega, pi, sigma, theta and zeta, each of which is composed of proteins that have a variety of functions throughout the cell. GSTM5 plays an important role in detoxification. Source: Recombinant protein corresponding to aa1-218 from human GSTM5, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~28.2kD (242aa), Specific Activity: ~90,000 pmol/min/ug, and is defined as the amount of enzyme that conjugate 1umole of 1-chloro-2,4- dinitrobenzene (CDNB) with reduced glutathione/minute at pH 6.5 at 25°C. Activity Assay: 1. Prepare a 180ul assay buffer into a suitable container. The concentrations are 106mM potassium phosphate (pH6.5), 1.1mM EDTA, 5.5mM glutathione, reduced, 2.2mM 1-chloro-2,4-dinitrobenzene (CDNB), 8% (v/v) ethanol., 2. Equilibrate to 25°C and monitor at A340nm until the value is constant using a spectrophotometer., 3. Add 20ul of recombinant GSTM5 protein 1.25ug/ml in assay buffer., 4. Mix by inversion and record the decrease at A340nm for approximately 5 minutes. AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMPMTLG YWDIRGLAHA IRLLLEYTDS SYVEKKYTLG DAPDYDRSQW LNEKFKLGLD FPNLPYLIDG AHKITQSNAI LRYIARKHNL CGETEEEKIR VDILENQVMD NHMELVRLCY DPDFEKLKPK YLEELPEKLK LYSEFLGKRP WFAGDKITFV, DFLAYDVLDM KRIFEPKCLD AFLNLKDFIS RFEGLKKISA YMKSSQFLRG LLFGKSATWN SK, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Keywords: | GSTM5, GSTM5-5, EC=2.5.1.18, GST class-mu 5, Glutathione S-transferase Mu 5 |
Supplier: | United States Biological |
Supplier-Nr: | 351270 |
Properties
Conjugate: | No |
Species reactivity: | human |
MW: | 28.2 kD |
Purity: | ~95% (SDS-PAGE) |
Format: | Highly Purified |
Database Information
KEGG ID : | K00799 | Matching products |
UniProt ID : | P46439 | Matching products |
Gene ID | GeneID 2949 | Matching products |
Handling & Safety
Storage: | -20°C (-70 °C for long term storage) |
Shipping: | +4°C (International: °C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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