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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
351268.100 | 100 µg | - | - |
3 - 19 business days* |
719.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
GSTA4, also known as glutathione S-transferase A4, belongs to the GST superfamily. This enzyme is... more
Product information "GSTA4, Recombinant, Human, aa1-222, His-Tag (Glutathione S-transferase alpha 4, GSTA4-4, GTA4)"
GSTA4, also known as glutathione S-transferase A4, belongs to the GST superfamily. This enzyme is involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. GSTA4 shows very high activity with reactive carbonyl compounds such as alk-2-enals. GSTA4 is highly effective in catalyzing the conjugate addition of reduced glutathione to 4-hydroxynonenal, an important product of peroxidative degradation of arachidonic acid and a commonly used biomarker for oxidative damage in tissue. This enzyme is expressed at a high level in brain, placenta, and skeletal muscle and much lower in lung and liver. Source: Recombinant protein corresponding to aa1-222 from human GSTA4, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~28.3kD (246aa), Specific Activity: ~4000 pmol/min/ug, and is defined as the amount of enzyme that conjugate 1umole of 1-chloro-2,4- dinitrobenzene (CDNB) with reduced glutathione/minute at pH 6.5 at 25°C. Activity Assay: 1. Prepare a 180ul assay buffer into a suitable container. The concentrations are 106mM potassium phosphate (pH6.5), 1.1mM EDTA, 5.5mM glutathione, reduced, 2.2mM 1-chloro-2,4-dinitrobenzene (CDNB), 8% (v/v) ethanol., 2. Equilibrate to 25°C and monitor at A340nm until the value is constant using a spectrophotometer., 3. Add 20ul of recombinant GSTA4 protein 40ug/ml in assay buffer., 4. Mix by inversion and record the decrease at A340nm for approximately 5 minutes. AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMAARPK LHYPNGRGRM ESVRWVLAAA GVEFDEEFLE TKEQLYKLQD GNHLLFQQVP MVEIDGMKLV, QTRSILHYIA DKHNLFGKNL KERTLIDMYV EGTLDLLELL IMHPFLKPDD QQKEVVNMAQ KAIIRYFPVF EKILRGHGQS FLVGNQLSLA, DVILLQTILA LEEKIPNILS AFPFLQEYTV KLSNIPTIKR FLEPGSKKKP PPDEIYVRTV YNIFRP, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Keywords: | GSTA4, EC=2.5.1.18, GST class-alpha member 4, Glutathione S-transferase A4, Glutathione S-transferase A4-4 |
Supplier: | United States Biological |
Supplier-Nr: | 351268 |
Properties
Conjugate: | No |
Species reactivity: | human |
MW: | 28.3 kD |
Purity: | ~95% (SDS-PAGE) |
Format: | Highly Purified |
Database Information
KEGG ID : | K00799 | Matching products |
UniProt ID : | O15217 | Matching products |
Gene ID | GeneID 2941 | Matching products |
Handling & Safety
Storage: | -20°C (-70 °C for long term storage) |
Shipping: | +4°C (International: °C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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