GITR Extracellular Domain (human, recombinant)

Glucocorticoid-induced TNFR-related protein (GITR), also known as TNF receptor superfamily member 18 (TNFRS18), is a type I transmembrane glycoprotein and member of the tumor necrosis factor receptor superfamily with roles in acquired and innate immunity. It is composed of an extracellular domain that contains a ligand binding site, a transmembrane domain, and a cytoplasmic domain that facilitates the induction of NF-kappaB signaling. GITR is primarily expressed in immature and mature T cells and natural killer (NK) cells but is also expressed at low levels in mast cells, eosinophils, B cells, macrophages, as well as non-lymphoid tissues, osteoclast precursor cells, keratinocytes, and retinal epithelial cells. Upon binding of its ligand GITRL (Cay-32018), GITR binds various TNF receptor-associated factors (TRAFs) and induces signaling in a cell type-specific manner. GITR protein levels are increased in macrophages and dendritic cells in a mouse model of T. gondii infection, and administration of an agonistic anti-GITR antibody increases pro-inflammatory cytokine production in peritoneal fluid and reduces chronic phase parasite burden in the same model. Protein levels of GITR are also increased on CD4+CD25+, CD4+CD25high, and CD4+CD25+CD127low/- regulatory T cells isolated from patients with systemic lupus erythematosus (SLE) compared with healthy controls. Intratumor, but not peripheral, administration of an agonistic anti-GITR antibody increases overall survival in a GL261 murine glioma model. Cayman's GITR Extracellular Domain (human, recombinant) protein can be used for ELISA. This protein consists of 147 amino acids, has a calculated molecular weight of 16 kDa, and a predicted N-terminus of Gln26 after signal peptide cleavage. By SDS-PAGE, under reducing conditions, the molecular mass of the protein is approximately 27 kDa due to apparent post-translational modifications.Synonyms: CD357, GITR-D, Glucocorticoid-induced TNFR-related Protein, TNF Receptor Superfamily Member 18, TNFRS18, Tumor Necrosis Factor Receptor Superfamily Member 18. Purity: >95% estimated by SDS-PAGE. Endotoxin Testing: <1.0 EU/µg, determined by the LAL endotoxin assay. Source: Active recombinant human C-terminal His-tagged GITR expressed in HEK293 cells. Amino Acids: 26-161. MW: 16 kDa. Formulation: (Request formulation change), Lyophilized from sterile PBS, pH 7.4.