Flt3 Ligand, Recombinant, Murine (Fms-like Tyrosine Kinase 3 Ligand, flk2 Ligand)

Human FLT3 ligand also stimulates the proliferation of cells expressing murine FLT-3 receptors. The effects of FLT-3 ligand are synergized by coexpression of G-CSF , GM-CSF , M-CSF , IL3 and SCF . FLT-3 ligand promotes long-term expansion and differentiation of human pro-B-cells in the presence of IL7 or in combination of IL7 and IL3 . Recombinant Murine Flt3-Ligand is a non-glycosylated, polypeptide chain containing 144 amino acids with a MW of 16.43kD. , Sequence:, The sequence of the first five N-terminal amino acids was determined and was found to be Thr-Gln-Asp-Cys-Ser. Dimers and Aggregates: < 1% as determined by silver-stained SDS-PAGE gel analysis. Protein Content: Protein quantitation was carried out by two independent methods: , 1. UV spectroscopy at 280nm. , 2. Analysis by RP-HPLC, using a standard solution of FLT3 as a Reference Standard. Biological Activity: rmFlt3-Ligand is fully biologically active when compared to standard. The activity is determined by the ability to generate CD11c+ splenic dendritic cells in vivo in C57B1/6-12 week female mice. To obtain the desired affect, mice were treated with approximately 25ug of flt3-ligand once daily for 9 consecutive days via intraperitoneal injection. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. For long-term storage, store below -20°C. Reconstitute to nominal volume by adding sterile ddH2O, 0.1% HSA or BSA and store below -20°C. Reconstituted product is stable for 12 months below -20°C. Avoid freeze-thaw cycles. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.