ENPEP / Aminopeptidase A Protein (His tag) (recombinant mouse)

Activity: Measured by its ability to cleave the fluorogenic peptide substrate, Glu-7-amido-4-methyl coumarin.The specific activity is > 200 pmoles/min/µg. Protein Construction: A DNA sequence encoding the mature form of mouse ENPEP (P16406) (Arg41-Pro945) was expressed, with a polyhistidine tag at the N-terminus. Sequence: Arg41-Pro945. Fusion tag: N-His Endotoxin: < 1.0 EU per µg of the protein as determined by the LAL method. Apparent Molecular Mass: 113 kDa. Protein function: ENPEP, also known as aminopeptidase A, is a member of the peptidase M1 family. Members of this family are involved in response to cadmium ion and proteolysis. They located in 6 components and are expressed in 26 plant structures. ENPEP is expressed by epithelial cells of the proximal tubule cells and the glomerulus of the nephron. It also can be detected in a variety of other tissues. ENPEP probably plays a role in regulating growth and differentiation of early B-lineage cells. It also may play a role in the catabolic pathway of the renin-angiotensin system. ENPEP is a zinc-dependent membrane-bound aminopeptidase that catalyzes the cleavage of glutamatic and aspartatic amino acid residues from the N-terminus of polypeptides. It degrades vasoconstricting angiotensin II into angiotensin III and therefore helps to regulate blood pressure. Protein function: Regulates central hypertension through its calcium- modulated preference to cleave N-terminal acidic residues from peptides such as angiotensin II. [The UniProt Consortium]