Cul1/Skp1/Skp2/Rbx1 (M5I) Complex Recombinant

Recombinant human complex of Cul1 (cullin 1), Skp1 (S-phase kinase associated protein 1), Skp2 (S-phase kinase associated protein2) and Rbx1 (Rbx1 (ring-box 1). Recombinant Cul1, encompassing amino acids 2-776(end), contains an N-terminal FLAG-Tag. Recombinant Skp1, encompassing amino acids 2-160 (end), contains an N-terminal His-Tag (6xHis). Recombinant Spk2, encompassing amino acids 2-424(end), contains an N-terminal FLAG-tag. Rbx1, full length, encompassing amino acids 2-108(end) contains an N-terminal His-tag (6xHis). These recombinant proteins are co-expressed in a HEK293 expression system and affinity purified.