Core Histones (human)

A nucleosome is the basic repeating unit of chromatin in which 146 base pairs of DNA wrap twice around a histone octamer consisting of two copies of each of the core histones, H2A, H2B, H3, and H4. The combination of two H2A/H2B dimers and one H3/H4 tetramer create the nucleosome core. Histone H3 undergoes many modifications which include acetylation, methylation, and phosphorylation that are important for regulation of gene transcription.Synonyms: HeLa Core Histones. Purity: >90% estimated by SDS-PAGE. Source: Highly purified mixture of human core histones (H2A, H2B, H3, and H4) isolated via hydroxyapatite chromatography from HeLa S3 (human cervical adenocarcinoma) nuclear pellet. Formulation: (Request formulation change), 50 mM sodium phosphate, pH 6.9, containing 10 mM sodium chloride and 1 mM beta-mercaptoethanol.