Citrullinated Nucleophosmin (human, recombinant)

Nucleophosmin is a nucleolar phosphoprotein that is involved in diverse biological processes, including ribosome maturation, DNA repair, and mitotic spindle assembly. It is highly post-translationally modified and composed of an oligomerization domain, which contains two nuclear export signals, three acidic domains, an intrinsically disordered region, which contains two nuclear localization signals, and a C-terminal RNA-binding domain. Nucleophosmin is ubiquitously expressed and shuttles between the nucleolus and cytoplasm, however, alternative splicing of NPM1 produces a variant that lacks the RNA-binding domain and nucleolar localization signal and is instead localized throughout the nucleus. Nucleophosmin has histone- and protein chaperone activity and plays a role in ribosome assembly and export, centrosome duplication, cell cycle control, the stress response, and embryogenesis. Nucleophosmin citrullinated by PAD4 increases p53 stability and radiation sensitivity in a thyroid cancer cell line exposed to radiation. This product contains purified nucleophosmin (Cay-37621) that has been modified with PAD4, which is subsequently depleted by affinity chromatography.Synonyms: NPM, NPM1, Nucleolar Phosphoprotein B23, Nucleolar Protein NO38, Numatrin. Purity: >60% estimated by SDS-PAGE. Source: Recombinant human N-terminal His-TEV-tagged NPM1 expressed in E. coli and citrullinated by PAD4. Amino Acids: 2-294 (full length). MW: 34.7 kDa. Formulation: (Request formulation change), 50 mM HEPES, pH 7.8, with 150 mM sodium chloride and 10% glycerol. UniProt Accession #: P06748.