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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
C2589-79C.50 | 50 µg | - | - |
3 - 19 business days* |
947.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
Carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM-6), previously called... more
Product information "CEACAM 6, Recombinant, Human (Carcinoembryonic Antigen Related Cell Adhesion Molecule)"
Carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM-6), previously called nonspecific crossreacting antigen (NCA) or CD66c, is one of seven human CEACAM family members within the immunoglobulin superfamily (1-4). In humans, CEACAMs include type I transmembrane proteins (CEACAM-1, -3, and -4) and GPI-linked molecules (CEACAM-5 through -8) (1). There is no human CEACAM-2. Human CEACAM-6 is a 90 kD, GPI-linked membrane protein that contains a 34 amino acid (aa) signal sequence, a 286 aa extracellular domain (ECD), and a 24 aa hydrophobic C-terminal propeptide. The GPI membrane anchor is attached at the C terminus following cleavage of the propeptide. CEACAM-6 contains one N-terminal V-type Ig-like domain (N domain), followed by two C2-type Ig-like domains (2-4). It shows considerable glycosylation, including (sialyl) Lewis X , which mediates binding to E-selectin, galectins and some bacterial fimbrae (1, 2). Mature human CEACAM-6 shows 84%, 85%, 80%, 87% and 51% aa identity to the equivalent extracellular regions of human CEACAMs 1, 5 (CEA) and 8, rhesus CEACAM-2, and bovine CEACAM-6, respectively. CEACAM-6 is expressed by granulocytes and their precursors. Activation enhances surface expression by mobilizing CEACAM-6 from storage in azurophilic granules (5, 6). It often shows aberrant expression in acute lymphocytic leukemias (10). CEACAM-6 is also expressed epithelia of various organs and is upregulated in pancreatic and colon adenocarcinomas and hyperplastic polyps (7, 8). Over-expression confers resistance to adhesion-related apoptosis (anoikis) in tumor cells (8, 9). CEACAM-6 is an intercellular adhesion molecule, forming both homotypic, and heterotypic bonds with CEACAM-1, -5 and -8 through interaction of the V-type Ig-like domains (11, 12). Cross-linking of neutrophil CEACAM-6 augments alpha vbeta3 and beta2 integrin-mediated adhesion, apparently by src and caveolin-mediated inside-out integrin activation (8, 13, 14). Source: Human CD33 Signal Peptide (Met 1-Ala 16) Human CEACAM-6 (Lys 35-Gly 320) HHHHHH. A DNA sequence encoding the mature human CEACAM-6 (Lys 35-Gly 320, Accession # P40199) (Barnett, T. et al., 1998, Genomics 3(1):59) was fused to the signal peptide from human CD33 at the amino terminus and to a polyhistidine tag at the carboxy-terminus. The chimeric protein was expressed in a mouse myeloma cell line, NS0. Molecular Mass: Based on N-terminal sequencing, the recombinant human CEACAM-6 starts at Lys 35 and has a calculated molecular mass of approximately 32.0kD. As a result of glycosylation, the recombinant protein migrates as an approximately 57-75kD protein in SDS-PAGE under reducing conditions. Endotoxin Level: < 1 EU/ug of the protein as determined by the LAL method. Activity: Measured by the ability of the immobilized protein to support the adhesion of 1 µM calcium ionosphere treated human neutrophils. When 2x10e5 cells/well are added to CEACAM-6-coated plates (10ug/ml, 100ul/well), 35-60% of the cells will adhere after 20 minutes at 37°C. Reconstitution: It is recommended that sterile PBS be added to the vial to prepare a working stock solution of no less than 100ug/ml. The carrier-free protein should be used immediately upon reconstitution to avoid losses in activity due to non-specific binding to the inside surface of the vial. For long term storage as a dilute solution, a carrier protein (e.g. 0.1% HSA or BSA) should be added to the vial. Storage: Lyophilized samples are stable for up to twelve months at -20°C. Upon reconstitution, this protein, in the presence of a carrier protein, can be stored under sterile conditions at 2-8°C for one month or at -20°C in a manual defrost freezer for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Keywords: | NCA, CD66c, CEACAM6, Normal cross-reacting antigen, Non-specific crossreacting antigen, Carcinoembryonic antigen-related cell adhesion molecule 6 |
Supplier: | United States Biological |
Supplier-Nr: | C2589-79C |
Properties
Conjugate: | No |
Species reactivity: | human |
Format: | Highly Purified |
Database Information
KEGG ID : | K06499 | Matching products |
UniProt ID : | P40199 | Matching products |
Gene ID | GeneID 4680 | Matching products |
Handling & Safety
Storage: | -20°C |
Shipping: | +4°C (International: +4°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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