CD70 IgG2a Fc, Fusion Protein (Tnfsf7) (Biotin)

Human CD70 (TNFSF7) is the ligand for CD27 (TNFRSF7) and is notably absent on resting lymphocytes. CD70 is expressed after activation on about 20% of T cells and on about 70% of B cells. Recombinant CD70-muCD8 binds to cell surface CD27 on human peripheral blood leukocytes. Molecular Structure:  A soluble molecule consisting of the extracellular domain (167aa) of murine CD8 alpha fused to 146 (c-term) amino acids of the extracellular domain of human CD70, with a predicted monomeric weight of 34.8kD. Extracellular mature murine CD8alpha: (28)KPQAPEL....GLDFACD(194), Linking amino acids: GT, extracellular human CD70: (48)LPLESL....VQWVRP(193). Applications: Suitable for use in ELISA and Flow Cytometry. Other applications not tested. Recommended Dilution Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.