CD226 Extracellular Domain (human, recombinant)

CD226, also known as DNAM-1, is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily. It is composed of an N-terminal signal peptide, an extracellular domain containing two immunoglobulin-like (Ig-like) domains and two paired cysteine residues, a transmembrane domain, and a C-terminal cytoplasmic region containing at least two phosphorylation sites important for ligand binding. It is expressed on certain T cells, natural killer (NK) cells, monocytes, and B cells and mediates cell adhesion between these and other immune cells. CD226 is an adhesion protein that binds to its ligands, PVR/CD155 or nectin-2/CD112, expressed on immature dendritic cells, as well as infected or transformed cells, to induce NK-mediated killing and cell lysis. CD226 also associates with lymphocyte function-associated antigen 1 (LFA1) on activated NK cells, which is essential for its signaling. CD226 is involved in immunological synapse formation and acts as a co-stimulatory molecule on NK cells to promote antigen presenting cell-induced cytokine release that, in turn, induces IFN-gamma secretion from NK cells. It is also involved in NK cell-mediated immunosurveillance, reducing tumor growth in mouse models of cancer while Cd226-/- mice are more susceptible to NK cell-dependent tumor initiation. A glycine-to-serine mutation at position 307 of CD226 is associated with autoimmune diseases, such as rheumatoid arthritis, systemic lupus erythematosus (SLE), and multiple sclerosis. Cayman's CD226 Extracellular Domain (human, recombinant) protein can be used for binding assay applications. This protein consists of 240 amino acids, has a calculated molecular weight of 27.6 kDa, and a predicted N-terminus of Glu19 after signal peptide cleavage. By SDS-PAGE, under reducing conditions, the apparent molecular mass of the protein is approximately 40 to 50 kDa due to glycosylation.Synonyms: Cluster of Differentiation 226, DNAX Accessory Molecule-1, DNAM-1, PTA1, TLISA1. Purity: >95% estimated by SDS-PAGE. Endotoxin Testing: <1.0 EU/µg determined by the LAL endotoxin assay. Source: Active recombinant human C-terminal His-tagged CD226 expressed in HEK293 cells. Amino Acids: 19-247. MW: 27.6 kDa. Formulation: (Request formulation change), Lyophilized from sterile PBS, pH 7.4. UniProt Accession #: Q15762.