Cathepsin G, Highly Purified (EC 3.4.21.20, Human Neutrophils)

Cathepsins are a group of intracellular proteolytic enzymes located within the lysosomes. On the basis of the specificity towards their substrates, the cathepsins have been assigned the names: Cathepsins A, B, C, D, E, G H and L. Cathepsin G is a serine protease, it consists of a single polypeptide with a MW of 30kD as determined by SDS-PAGE. Its substrates are collagen and proteoglycans. Cathepsins are localized primarily in lysosomes or endosomes, but translocated to the cytosol during apoptosis. The translocation allows these proteases to interact with caspases or other apoptosis signaling mechanisms. Studies show that cathepsins cleave certain caspases, and caspase inhibitors can inhibit cathepsin activity in vitro. Applications: Suitable for use as an antigen in immunological protocols. Biological Activity: 2-4 Units/mg, Unit Definition: 1unit of activity is defined as the amount of enzyme that hydrolyzes 1uM per minute. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C.For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.