Cathepsin B, C-terminal HIS tag, Recombinant, Human (Procathepsin B, Cathepsin B1, APP secretase, AP

Source:, Recombinant corresponding to CD33 signal peptide(aa1-16) and human cathepsinB (aa18-339) with C-terminal HIS tag expressed in freestyle 293-F cells (NM_001908). Specific Activity: 2228pmol/min/ng Assay condition : 25mM MES, pH 5.0, 10uM substrate (Z-Leu-Arg-AMC), 10 minutes at RT. Prior to assay enzyme was activated with preincubation at 10ug/ml in 25mM MES, pH 5.0, 5mM DTT for 15 minute at RT. Applications: Suitable for use in the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing. Store at -70°C. Aliquots are stable for at least 6 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.