Catalase, Suspension (EC 1.11.1.6, Bovine liver)

Catalase is a hemoprotein containing four subunits which decomposes peroxide. Catalytic activity is present in nearly all animal cells and organs and in aerobic microorganisms. Activity: >20,000 units/mg protein, Unit Definition: 1 unit decomposes 1umole of hydrogen peroxide/minute at 25°C, pH 7.0. Composition: 4 subunits of equal size (MW 60,000-65,000), Quality Control: SDS-PAGE, Handling: Contains thymol as a preservative. To remove thymol, measure desired amount of mixed suspension, centrifuge to collect enzyme crystals, remove supernatant. Resuspend crystals in one-half initial volume of water, respin. Discard wash supernatant and dissolve crystals in buffer of choice. Storage and Stability: May be stored at 4°C. Do not freeze. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.