Calsarcin-2, Mouse, Control Peptide (Myozenin-1)

Control Peptide for (affinity purified) and (antiserum)., Synthetic peptide, 17-aa peptide, ~N-terminus. Calcineurin is a Ca/calmodulin-dependent serine-threonine phosphatase that plays an important role in transducing Ca-dependent signals in a variety of cell types. Calcineurin has also been shown to have a profound influence on the properties of striated muscle cells, including cardiac muscle. A novel family of striated muscle-specific calcineurin-interacting proteins called calsarcins or Myozenins has been identified that interact and colocalizes with the Z-disc protein alpha-actinin. Two isoform of calsarcins, Calsarcin-1 and Calsarcin-2, with specific expression pattern have been identified in human, rat and mouse. Calsarcins tether calcineurin to the sarcomere of cardiac and skeletal muscle. Besides calcineurin and a-actinin, calsarcins interact with other Z-disc proteins g-filamin, telethonin and TCAP. Because calcineurin responds to sustained, low amplitude calcium signals, calsarcins may serve to localize calcineurin in the vicinity of unique intracellular pool, where it can interact with specific upstream activators or downstream substrates. Therefore, calsarcins may play an important role in modulating the function and substrate specificity of calcineurin in striated muscle cells. , Calsarcin-2/CALS-2 (variouslytermed Myozenin-1 and FATZ) ~34kD, human 299-aa, mouse 296-aa, ~30% identity with CALS-1) is a globular protein with central glycine-rich domain flanked by a-helical regions. CALS-2 is expressed transiently in heart during early embryogenesis and later becomes restricted to skeletal muscle with weaker signals in adult prostate, placenta and pancreas. In contrast to CALS-1, the expression of Calsarcin-2 is restricted to fast-twitch skeletal fiber (Gastrocnemius), which receives intermittent, high frequency stimulation and exhibit lower intracellular calcium concentration. Calsarcin-2 interacts with other Z-disc proteins g-Filamin, ACTN-2, ACTN-3 and TCAP and possibly modulates both g-filamin and a-actinin dimerization. Based on its binding partners, Calsarcin-2 has been suggested to play a central role in myofibrillogenesis and as a candidate for limb-girdle muscular dystrophy and other related neuromuscular disorders. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.