BRSK2, N-terminal His tag, Recombinant, Human (BR Serine/threonine Kinase 2, SAD1, Homolog of SAD-1

Source:, Recombinant corresponding to full length human BRSK2 with N-terminal His tag expressed in baculovirus expression system (NM_003957). Specific Activity: 412pmoles/min/ug. Enzyme reaction is conducted in a buffer containing 50mM HEPES, pH 7.5, 10mM MgCl2, 1mM EGTA, 200uM ATP, 0.01% Brij-35 and 2uM substrate (SER/THR 21) at RT for 1 hour. Applications: Suitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing. Store at -70°C. Aliquots are stable for at least 6 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.