beta-Acetylglucosaminidase 18A, Bacillus megaterium

beta-Acetylglucosaminidase 18A (BmAcp18A), assigned the E.C. number 3.2.1.-, is a derivative of Bacillus megaterium. It is a cortex peptidoglycan hydrolase with endo-beta-N-acetylglucosaminidase specificity responsible for cortex peptidoglycan degradation that triggers spore germination. The recombinant BmAcp18A, purified from Escherichia coli, is a modular Glycoside Hydrolase family 18 (GH18) enzyme, with two N-terminal family 50 Carbohydrate Binding Modules (CBM50) (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 0,25 mg/mL. Bulk quantities of this product can be made available upon request. Shipping Conditions: Shipping: Dry Ice to RT. Storage Conditions: -30°C to -15°C. Class: Glycoside Hydrolase. Activity: Acetylglucosaminidases. Family: GH18. Ec Number: 3.2.1.-. Organism: Bacillus megaterium. Architecture: CBM50-CBM50-GH18. Optimal pH: 7. Optimal Temperature: 37°C. Substrates: spore cortex peptidoglycan