Anti-MERS-CoV Nucleocapsid Protein (recombinant)

Middle East respiratory syndrome coronavirus (MERS-CoV) is an enveloped positive-stranded RNA virus, a member of the Betacoronavirus genus, and a causative agent of MERS, an acute respiratory disease that often leads to pneumonia and renal failure. The MERS-CoV nucleocapsid protein is composed of an intrinsically disordered region (IDR), an N-terminal domain that binds RNA, a flexible linker containing serine and arginine phosphorylation sites, a C-terminal domain that also participates in RNA binding and is responsible for dimerization, and another IDR. Coronavirus nucleocapsid proteins, including the MERS-CoV nucleocapsid protein, package the viral genome into a ribonucleoprotein (RNP) complex. In addition, the MERS-CoV nucleocapsid protein interacts with the ubiquitin ligase TRIM25 and inhibits the activation of retinoic acid-inducible gene I (RIG-I) and phosphorylation of IFN regulatory factor 3 (IRF3) and NF-kappaB. It reduces IFN-beta and IFN-lambda1 promoter activity induced by the RIG-I caspase activation and recruitment domain (RIG-I-CARD), but not MDA5-CARD, and reduces Sendai virus-induced increases in IFN-beta and IFN-lambda1 mRNA levels. Cayman's MERS-CoV Nucleocapsid Protein (recombinant) consists of 424 amino acids and has a calculated molecular weight of 46.51 kDa.Synonyms: MERS-CoV Nucleoprotein, MERS-CoV N Protein. Purity: >90% as estimated by SDS-PAGE. Endotoxin Testing: <1.0 EU/µg, determined by the LAL endotoxin assay. Source: Recombinant MERS-CoV C-terminal His-tagged nucleocapsid expressed in insect cells. Amino Acids: 1-413 (full length). MW: 46.51 kDa. Formulation: (Request formulation change), Lyophilized from sterile 20 mM Tris, pH 8.0, with 500 mM sodium chloride and 10% glycerol. Host: Insect cells. UniProt Accession #: K0BVN3.