Annexin V (human, rec.) (ATTO 488)

Recombinant human annexin V fused to a GST-tag. Liquid. In PBS, pH 7.4, containing 0.02% sodium azide. Binds to phosphatidylserine (PS). Application: This protein can be used for the detection of apoptotic cells by flow cytometry or immunofluorescence (100 tests/0.5ml). Annexin V is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. Annexin V competes for phosphatidylserine binding sites (e.g. platelets) with prothrombin or coagulation factors and inhibis the activity of phospholipase A1. Antibodies directed against annexin V are found in patients with a disease called the antiphospholipid syndrome(APS), a thrombophilic disease associated with autoantibodies against phospholipid compounds. Annexin V is used as a probe in the 'annexin V affinity assay' to detect cells that have expressed phosphatidylserine on the cell surface, a feature found in apoptosis and other forms of cell death.