Angiopoietin-like Protein 3, Recombinant, Human (ANGPTL3)

Angiopoietin-like proteins ANGPTL3 and ANGPTL4 are secreted proteins mainly expressed in liver that have been demonstrated to regulate triglyceride metabolism by inhibiting the lipolysis of triglyceride-rich lipoproteins. ANGPTL3 is structurally similar to angiopoietins, which are vascular endothelial growth factors. The experimental results show that Angptl3 and Angptl4 function to regulate circulating triglyceride levels during different nutritional states and therefore play a role in lipid metabolism during feeding/fasting through differential inhibition of Lipoprotein lipase (LPL). , g deletion mutants of human ANGPTL3, it was demonstrated that the N-terminal domain containing fragment-(17-207) and not the C-terminal fibrinogen-like domain containing fragment-(207-460) increased the plasma triglyceride levels in mice. , Recombinant Human ANGPTL3 produced with N-terminal fusion of HisTag, is a 26kD polypeptide containing 207 amino acid residues of the Human ANGPTL3 and 16 additional amino acid residues. Amino Acid sequence: MRGSHHHHHH GMASHMSRID QDNSSFDSLS PEPKSRFAML DDVKILANGL LQLGHGLKDF VHKTKGQIND IFQKLNIFDQ SFYDLSLQTS EIKEEEKELR RTTYKLQVKN EEVKNMSLEL NSKLESLLEE KILLQQKVKY LEEQLTNLIQ NQPETPEHPE VTSLKTFVEK QDNSIKDLLQ TVEDQYKQLN QQHSQIKEIE NQLRRTSIQE PTEISLSSKP RAP. Applications: Suitable for use in ELISA and Western Blot. Other applications have not been tested. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.