Acrp30, soluble, Recombinant, Human (Adipocyte Complement-related Protein of 30kD, Adiponectin, Adip

Adipose tissue is the largest reservoir of fuel, storing energy in the form of rapidly utilizable triglycerides. Adipocytes synthesize and store energy in periods of nutritional abundance and mobilize lipids during starvation and other times of need. In order to accomplish these complex tasks of energy balance, adipocytes express many genes, including Acrp30 which is involved in lipid metabolism and glucose homeostasis. Acrp30 (Adipocyte complement-related protein of 30kD), also known as AdipoQ, APM1, Adiponectin, Gelatin binding protein 28kD/GBP28 or adipocyte most abundant gene transcript) was identified as a novel adipocyte-specific synthesized. It is a secreted protein with structural resemblance to complement factor C1q. Like adipsin, Acrp30 secretion is induced ~10-fold during adipocyte differentiation. Plasma levels are reduced in obese humans. Low levels are associated with insulin-resistance. Treatment of db/db mice with TZD increased Acrp30 levels. Acrp30 (mouse 247aa, rat human 244aa, chromosome 3q27) consists of a predicted NT-signal sequence 91-14aa), followed by a 27aa unique region, followed by 22 perfect Gly-X-Pro or Gly-X-X collagen like repeats, and a globular segment at the C-terminus. Structurally, at the sequence level, Acrp30 resembles other collagen-like and globular domain proteins (lung surfactant protein and hepatocytes mannan-binding proteins). Acrp30 is proteolytically cleaved at 104aa to generate the globular Acrp30 (gAcrp30). Administration of gAcrp30 into mice fed a diet high in fat and sugar caused substantial weight loss. A marked reduction in plasma triglycerides, glucose and free fatty acids was attributed due in part to increased fatty acid oxidation by muscle. Full length Acrp30 was less potent than gAcrp30. Therefore, gAcrp30 may open new avenues to control obesity. Human ACRP30 (aa16-244) is fused at the N-terminus to a linker peptide (14aa) and a FLAG tag. MW: ~33kD (SDS-PAGE under reducing conditions). Mimics serum adiponectin by forming high molecular weight (HMW) and hexameric species. Biological Activity:, Activates AMPK, Application: Inhibits serum glucose production in mice. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute with ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made with medium containing 5% fetal calf serum or other carrier protein.