SAMS Peptide

AMP-activated protein kinase (AMPK) plays a role in energy metabolism and glucose homeostasis by monitoring the ratio of ATP to AMP. This kinase can inactivate HMG-CoA reductase and acetyl-CoA carboxylase as well as block the activation of hormone-sensitive lipase by cyclic-AMP-dependent protein kinase (protein kinase A or PKA). SAMS peptide is a synthetic peptide substrate for AMPK based on the sequence around Ser79 (which is phosphorylated exclusively by AMPK) on acetyl-CoA carboxylase. In this sequence, Ser77 (which is phosphorylated exclusively by PKA) is replaced by alanine to eliminate a neighboring binding site for PKA. This substitution yields a substrate more specific for AMPK than acetyl-CoA carboxylase itself. SAMS peptide is phosphorylated rapidly by AMPK, providing a convenient and sensitive tool for assaying AMPK activity.Formal Name: L-histidyl-L-methionyl-L-arginyl-L-seryl-L-alanyl-L-methionyl-L-serylglycyl-L-leucyl-L-histidyl-L-leucyl-L-valyl-L-lysyl-L-arginyl-L-arginine. CAS Number: 125911-68-4. Molecular Formula: C74H131N29O18S2. Formula Weight: 1779.2. Purity: >95%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 15 mg/ml, DMSO: 15 mg/ml, Ethanol: 1 mg/ml, PBS (pH 7.2): 10 mg/ml. SMILES: [H]N[C@H](C(N[C@H](C(N[C@@H](CCCNC(N)=N)C(N[C@@H](CO)C(N[C@H](C(N[C@H](C(N[C@@H](CO)C(NCC(N[C@@H](CC(C)C)C(N[C@H](C(N[C@@H](CC(C)C)C(N[C@@H](C(C)C)C(N[C@@H](CCCCN)C(N[C@@H](CCCNC(N)=N)C(N[C@@H](CCCNC(N)=N)C(O)=O)=O)=O)=O)=O)=O)CC1=CN=CN1)=O)=O)=O)=O)CCSC)=O)C)=O)=O)=O)CCSC)=O)CC2=CN=CN2. InChi Code: InChI=1S/C74H131N29O18S2/c1-38(2)27-51(66(115)100-53(30-43-32-84-37-90-43)67(116)99-52(28-39(3)4)68(117)103-57(40(5)6)70(119)97-45(15-10-11-21-75)61(110)95-46(16-12-22-85-72(77)78)62(111)98-50(71(120)121)18-14-24-87-74(81)82)92-56(106)33-88-60(109)54(34-104)101-65(114)48(19-25-122-8)93-58(107)41(7)91-69(118)55(35-105)102-63(112)47(17-13-23-86-73(79)80)96-64(113)49(20-26-123-9)94-59(108)44(76)29-42-31-83-36-89-42/h31-32,36-41,44-55,57,104-105H,10-30,33-35,75-76H2,1-9H3,(H,83,89)(H,84,90)(H,88,109)(H,91,118)(H,92,106)(H,93,107)(H,94,108)(H,95,110)(H,96,113)(H,97,119)(H,98,111)(H,99,116)(H,100,115)(H,101,114)(H,102,112)(H,103,117)(H,120,121)(H4,77,78,85)(H4,79,80,86)(H4,81,82,87)/t41-,44-,45-,46-,47-,48-,49-,50-,51-,52-,53-,54-,55-,57-/m0/s1. InChi Key: YJAHUEAICBOBTC-CSVPVIMBSA-N.