Ac-VEID-AMC (ammonium acetate salt)

Ac-VEID-AMC is a fluorogenic substrate based on the caspase-6 cleavage site in lamin A at amino acids VEID during apoptosis. It has also been reported to be cleaved by related proteases, including caspase-8. Caspase activity can be quantified by fluorescent detection of free AMC (also known as 7-amino-4-methylcoumarin), which is excited at 340-360 nm and emits at 440-460 nm.Formal Name: N-acetyl-L-valyl-L-alpha-glutamyl-L-isoleucyl-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)-L-alpha-asparagine, ammonium acetate salt. Synonyms: N-Acetyl-Val-Glu-Ile-Asp-7-amido-4-Methylcoumarin, Caspase-6 Substrate (Fluorogenic). Molecular Formula: C32H43N5O11 . XC2H7NO2. Formula Weight: 673.7. Purity: >95%. Emission: 440-460 nm. Excitation: 340-360 nm. Formulation: (Request formulation change), A crystalline solid. Solubility: DMSO: 10 mg/ml. lambdamax: 328 nm. SMILES: CC(C1=CC=C(C=C1O2)NC([C@@H](NC(C(NC([C@@H](NC([C@@H](NC(C)=O)C(C)C)=O)CCC(O)=O)=O)[C@H](CC)C)=O)CC(O)=O)=O)=CC2=O.CC([O-])=O.[NH4+]. InChi Code: InChI=1S/C32H43N5O11.C2H4O2.H3N/c1-7-16(4)28(37-29(44)21(10-11-24(39)40)35-31(46)27(15(2)3)33-18(6)38)32(47)36-22(14-25(41)42)30(45)34-19-8-9-20-17(5)12-26(43)48-23(20)13-19,1-2(3)4,/h8-9,12-13,15-16,21-22,27-28H,7,10-11,14H2,1-6H3,(H,33,38)(H,34,45)(H,35,46)(H,36,47)(H,37,44)(H,39,40)(H,41,42),1H3,(H,3,4),1H3/t16-,21-,22-,27-,28?,,/m0../s1. InChi Key: KSHXSSVLIQDWNA-OOJFZAAOSA-N.