Ac-LETD-AFC

Ac-LETD-AFC is a fluorogenic substrate that can be cleaved specifically by caspase-8. Caspase activity can be quantified by fluorescent detection of free AFC (also known as 7-amino-4-trifluoromethylcoumarin), which is excited at 400 nm and emits at 505 nm.Formal Name: N-acetyl-L-leucyl-L-alpha-glutamyl-L-threonyl-N-[2-oxo-4-(trifluoromethyl)-2H-1-benzopyran-7-yl]-L-alpha-asparagine. CAS Number: 210345-02-1. Synonyms: N-Acetyl-Leu-Glu-Thr-Asp-7-amino-4-Trifluoromethylcoumarin, Caspase-8 Substrate (Fluorogenic). Molecular Formula: C31H38F3N5O12. Formula Weight: 729.7. Purity: >95%. Emission: 505 nm. Excitation: 400 nm. Formulation: (Request formulation change), A crystalline solid. Solubility: DMSO: 10 mg/ml. lambdamax: 206, 339 nm. SMILES: OC(C[C@H](NC(C(NC([C@@H](NC([C@H](CC(C)C)NC(C)=O)=O)CCC(O)=O)=O)[C@@H](C)O)=O)C(NC(C=C1O2)=CC=C1C(C(F)(F)F)=CC2=O)=O)=O. InChi Code: InChI=1S/C31H38F3N5O12/c1-13(2)9-20(35-15(4)41)29(49)37-19(7-8-23(42)43)27(47)39-26(14(3)40)30(50)38-21(12-24(44)45)28(48)36-16-5-6-17-18(31(32,33)34)11-25(46)51-22(17)10-16/h5-6,10-11,13-14,19-21,26,40H,7-9,12H2,1-4H3,(H,35,41)(H,36,48)(H,37,49)(H,3. InChi Key: KTZOWCXXAYFIFT-XSSXFGQWSA-N.