PAR2 (1-6) amide (human) (trifluoroacetate salt)

PAR2 (1-6) amide is a synthetic peptide agonist of proteinase-activated receptor 2 (PAR2) that corresponds to residues 1-6 of the amino terminal tethered ligand sequence of human PAR2 and residues 37-42 of the full-length sequence. It binds to NCTC 2544 cells expressing human PAR2 (Ki = 9.64 µM in a radioligand binding assay) and induces calcium mobilization in the same cells (EC50 = 0.075 µM). PAR2 (1-6) amide (100 µM) reduces colony formation of A549 lung cancer cells. It induces superoxide production and degranulation in isolated human eosinophils when used at a concentration of 500 µM. PAR2 (1-6) amide (5 µmol/kg) induces tear secretion in rats when used in combination with amastatin (Cay-16719).Formal Name: L-seryl-L-leucyl-L-isoleucylglycyl-L-lysyl-L-valinamide, trifluoroacetate salt. CAS Number: 2379569-17-0. Synonyms: SLIGKV-NH2. Molecular Formula: C28H54N8O7 . XCF3COOH. Formula Weight: 614.8. Purity: >95%. Formulation: (Request formulation change), A solid. Solubility: DMF: 30 mg/ml, DMSO: 15 mg/ml, Ethanol: 5 mg/ml, PBS (pH 7.2): 10 mg/ml. SMILES: OC[C@H](N)C(N[C@@H](CC(C)C)C(N[C@@H]([C@@H](C)CC)C(NCC(N[C@@H](CCCCN)C(N[C@H](C(N)=O)C(C)C)=O)=O)=O)=O)=O.FC(F)(C(O)=O)F. InChi Code: InChI=1S/C28H54N8O7.C2HF3O2/c1-7-17(6)23(36-27(42)20(12-15(2)3)34-25(40)18(30)14-37)28(43)32-13-21(38)33-19(10-8-9-11-29)26(41)35-22(16(4)5)24(31)39,3-2(4,5)1(6)7/h15-20,22-23,37H,7-14,29-30H2,1-6H3,(H2,31,39)(H,32,43)(H,33,38)(H,34,40)(H,35,41)(H,36,42),(H,6,7)/t17-,18-,19-,20-,22-,23-,/m0./s1. InChi Key: HZTZARICKSBEFA-HZIHITNPSA-N.