Ac-LEHD-pNA (trifluoroacetate salt)

Ac-LEHD-pNA is a substrate for caspase-9. Caspase-9 preferentially binds to and cleaves the Leu-Glu-His-Asp (LEHD) peptide sequence to release p-nitroalinide, which can be quantified by colorimetric detection at 405 nm as a measure of enzyme activity.Formal Name: (2S,5S,8S,11S)-5-((1H-imidazol-5-yl)methyl)-8-(2-carboxyethyl)-11-isobutyl-2-(2-((4-nitrophenyl)amino)-2-oxoethyl)-4,7,10,13-tetraoxo-3,6,9,12-tetraazatetradecanoic acid, 2,2,2-trifluoroacetate. Synonyms: Ac-Leu-Glu-His-Asp-pNA, Caspase-9 Chromogenic Substrate I. Molecular Formula: C29H38N8O11 . XCF3COOH. Formula Weight: 674.7. Purity: >95%. Formulation: (Request formulation change), A lyophilized powder. Solubility: Water: 1 mg/ml. SMILES: CC(C)C[C@@H](C(N[C@H](C(N[C@@H](CC1=CN=CN1)C(N[C@@H](CC(NC2=CC=C([N+]([O-])=O)C=C2)=O)C(O)=O)=O)=O)CCC(O)=O)=O)NC(C)=O.OC(C(F)(F)F)=O. InChi Code: InChI=1S/C29H38N8O11.C2HF3O2/c1-15(2)10-21(32-16(3)38)27(43)34-20(8-9-25(40)41)26(42)35-22(11-18-13-30-14-31-18)28(44)36-23(29(45)46)12-24(39)33-17-4-6-19(7-5-17)37(47)48,3-2(4,5)1(6)7/h4-7,13-15,20-23H,8-12H2,1-3H3,(H,30,31)(H,32,38)(H,33,39)(H,34,43)(H,35,42)(H,36,44)(H,40,41)(H,45,46),(H,6,7)/t20-,21-,22-,23-,/m0./s1. InChi Key: YWFBXKIYWWARIC-JSEXCYGQSA-N.