Interferon alpha-2 (IFNA2) (Active), recombinant human

Organism: Homo sapiens (Human). Source: Mammalian cell. Expression Region: 24-188aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: CDLPQTHSLG SRRTLMLLAQ MRRISLFSCL KDRHDFGFPQ EEFGNQFQKA ETIPVLHEMI QQIFNLFSTK DSSAAWDETL LDKFYTELYQ QLNDLEACVI QGVGVTETPL MKEDSILAVR KYFQRITLYL KEKKYSPCAW EVVRAEIMRS FSLSTNLQES LRSKE. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: Less than 1.0 EU/ug as determined by LAL method. Biological Activity: 1Measured by its binding ability in a functional ELISA. Immobilized Human IFNA2 at 2 µg/mL can bind Human IFNAR2(CSB-MP011047HU). The EC50 is 154.2-191.9 ng/mL. 2Measured by its binding ability in a functional ELISA. Immobilized Human IFNA2 at 2 µg/mL can bind Anti-IFNA2 recombinant antibody(CSB-RA365593MA1HU). The EC50 is 2.366-2.818 ng/mL. Form: Lyophilized powder. Buffer: Lyophilized from a 0.2 µm filtered PBS, 6% Trehalose, pH 7.4. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: Produced by macrophages, IFN-alpha have antiviral activities. Reference: IFNA2 p.Ala120Thr impairs the inhibitory activity of Interferon-alpha2 against the hepatitis B virus through altering its binding to the receptor. Chen C., Zhu X., Xu W., Yang F., Zhang G., Wu L., Zheng Y., Gao Z., Xie C., Peng L. Antiviral Res 147:11-18 (2017). Function: nan