IL-1 alpha (human), animal-free recombinant

Product Description: IL-1alpha is a proinflammatory cytokine that is not secreted, it is produced by various cells like monocytes, tissue macrophages, keratinocytes, and other epithelial cells. Both IL-1alpha and IL-1beta bind to the same receptor and share similar, if not identical, biological properties. These cytokines have diverse effects, including stimulating thymocyte proliferation by triggering IL-2 release, fostering B-cell maturation and proliferation, exhibiting mitogenic FGF-like activity, and prompting the release of prostaglandin and collagenase from synovial cells. However, there is a notable difference: IL-1beta is secreted, while IL-1alpha primarily remains associated with cells. The recombinant Human IL-1alpha is an 18.0 kDa protein comprising 159 amino acid residues. . Amino acid sequence: SAPFSFLSNV KYNFMRIIKY EFILNDALNQ SIIRANDQYL TAAALHNLDE AVKFDMGAYK SSKDDAKITV ILRISKTQLY VTAQDEDQPV LLKEMPEIPK TITGSETNLL FFWETHGTKN YFTSVAHPNL FIATKQDYWV CLAGGPPSIT DFQILENQA. Endotoxin concentration: <0.1 EU/µg. Activity: Determined by its ability to stimulate the proliferation of mouse D10S cells. The expected ED50 is =0.001 ng/ml, corresponding to a specific activity of >1 x 10^9 units/mg. Contains no preservative. Protein function: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. [The UniProt Consortium]