Fibroblast Growth Factor 19, active (FGF19), Recombinant, Human

Involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. Stimulates glucose uptake in adipocytes. Activity requires the presence of KLB and FGFR4. {ECO:0000269, PubMed:12815072, ECO:0000269, PubMed:16597617, ECO:0000269, PubMed:17623664, ECO:0000269, PubMed:19085950}. Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 150ng/ml, corresponding to a specific activity of ?6.7x10e3 IU/mg. Sequence: MRPLAFSDAG PHVHYGWGDP IRLRHLYTSG PHGLSSCFLR IRADGVVDCA RGQSAHSLLE IKAVALRTVA IKGVHSVRYL CMGADGKMQG LLQYSEEDCA FEEEIRPDGY NVYRSEKHRL PVSLSSAKQR QLYKNRGFLP LSHFLPMLPM VPEEPEDLRG HLESDMFSSP LETDSMDPFG LVTGLEAVRS PSFEK, Molecular Weight: 21.8kD, PubMed ID: 9931477, 10525310, 12975309, 15489334, 15340161, 12815072, 16597617, 17623664, 18829467, 19085950, 20094046, 14730967, 17339340, Gene Name: FGF19, UNQ334/PRO533, Swiss Prot: O95750, Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.