Primers

Primers are short oligonucleotides, that usually consist of 20 to 40 nucleotides. In molecular biology, primers are mostly used for PCR (Polymerase Chain Reaction), that allows the amplification of DNA in vitro. The amplification is achieved by DNA-polymerases, to which the primers serve as starting points during DNA-synthesis. Primers can be used to obtain specific DNA-fragments for cloning experiments or, in the form of qRT-PCR (quantitative Real-Time PCR), for quantitative detection of nucleic acids.

Primers are short oligonucleotides, that usually consist of 20 to 40 nucleotides . In molecular biology, primers are mostly used for PCR (Polymerase Chain Reaction), that allows the... read more »
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Primers

Primers are short oligonucleotides, that usually consist of 20 to 40 nucleotides. In molecular biology, primers are mostly used for PCR (Polymerase Chain Reaction), that allows the amplification of DNA in vitro. The amplification is achieved by DNA-polymerases, to which the primers serve as starting points during DNA-synthesis. Primers can be used to obtain specific DNA-fragments for cloning experiments or, in the form of qRT-PCR (quantitative Real-Time PCR), for quantitative detection of nucleic acids.

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IL8, Human interleukin 8, Real Time PCR Primer Set
IL8, Human interleukin 8, Real Time PCR Primer Set

Item number: VHPS-4563

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: NAF, IL8, IL-8, MDNCF, LYNAP, NAP-1, GCP-1, CXCL8, MONAP, MDNCF-a, MDNCF-b, MDNCF-c, Emoctakin, IL-8(5-77), IL-8(6-77),...
Application: RNA quantification
43.00€ *
Review
Actb, Mouse actin, beta, Real Time PCR Primer Set
Actb, Mouse actin, beta, Real Time PCR Primer Set

Item number: VMPS-96

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: Actb, Beta-actin, Actin, cytoplasmic 1, Actin, cytoplasmic 1, N-terminally processed
Application: RNA quantification
43.00€ *
Review
Il6, Mouse interleukin 6, Real Time PCR Primer Set
Il6, Mouse interleukin 6, Real Time PCR Primer Set

Item number: VMPS-3096

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: Il6, mCG_11634, Interleukin 6, Interleukin 6, isoform CRA_b
Application: RNA quantification
43.00€ *
Review
Human MEGImmune Primer Library
Human MEGImmune Primer Library

Item number: HMEGIMM

Masterplate for SYBR green based Real Time PCR. Contains 374 primer sets directed against human calcium regulation related genes and 10 housekeeping gene primer sets. Provided in a 96-well microplate (10 µl - 10 µM). Perform up to 100 PCR arrays (based on 10 µl assay volume per reaction). Just add cDNA template and...
Keywords: HMEGIMM
Application: Gene expression profiling, PCR array
2,924.00€ *
Review
Human MEGoptosis Primer Library
Human MEGoptosis Primer Library

Item number: HMEGOP-1

Masterplate for SYBR green based Real Time PCR. Contains 374 primer sets directed against human calcium regulation related genes and 10 housekeeping gene primer sets. Provided in a 96-well microplate (10 µl - 10 µM). Perform up to 100 PCR arrays (based on 10 µl assay volume per reaction). Just add cDNA template and...
Keywords: HMEGOP-I
Application: Gene expression profiling, PCR array
2,924.00€ *
Review
18S, Human 18S Ribosomal RNA gene, Real Time PCR Primer Set
18S, Human 18S Ribosomal RNA gene, Real Time PCR Primer Set

Item number: VHPS-10350

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: qPCR, gene expression analysis
Application: RNA quantification
43.00€ *
Review
B2M, Mouse beta-2 microglobulin, Real Time PCR Primer Set
B2M, Mouse beta-2 microglobulin, Real Time PCR Primer Set

Item number: VMPS-563

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: B2m, Beta-2 microglobulin
Application: RNA quantification
43.00€ *
Review
HCRTR1, Human hypocretin (orexin) receptor 1, Real Time PCR Primer Set
HCRTR1, Human hypocretin (orexin) receptor 1, Real Time...

Item number: VHPS-4015

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: Ox1R, Ox1-R, Ox-1-R, HCRTR1, Orexin receptor type 1, Hypocretin receptor type 1
Application: RNA quantification
43.00€ *
Review
HCRTR2, Human hypocretin (orexin) receptor 2, Real Time PCR Primer Set
HCRTR2, Human hypocretin (orexin) receptor 2, Real Time...

Item number: VHPS-4016

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: Ox2R, Ox2-R, Ox-2-R, HCRTR2, Orexin receptor type 2, Hypocretin receptor type 2
Application: RNA quantification
43.00€ *
Review
IL1B, Human interleukin 1, beta, Real Time PCR Primer Set
IL1B, Human interleukin 1, beta, Real Time PCR Primer Set

Item number: VHPS-4531

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: IL1B, IL1F2, IL-1 beta, Catabolin, Interleukin-1 beta
Application: RNA quantification
43.00€ *
Review
VEGF, Human, Real Time PCR Primer Set
VEGF, Human, Real Time PCR Primer Set

Item number: VHPS-9899

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: VPF, VEGF, VEGFA, VEGF-A, Vascular permeability factor, Vascular endothelial growth factor A
Application: RNA quantification
43.00€ *
Review
Rat Housekeeping Genes Primer Sets
Rat Housekeeping Genes Primer Sets

Item number: RHK-1

Includes primer sets for Actb, B2m, Gapd, Gusb, Hprt1, Pgk, Pp1a, Rpl13a, Tbp and Tfrc. Normalize real time PCR results to multiple housekeepers rather than one for more accurate quantitation. Perform up to 1000 assays (based on 20 µl assay volume) for each housekeeping gene.
Application: RT-PCR, Gene expression analysis
138.00€ *
Review
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