CAY10665

Isolated neonatal rat cardiomyocytes, which spontaneously beat in culture, are used to evaluate the antiarrhythmic effects of polyunsaturated fatty acids (PUFA). In this model, the omega-3 PUFA eicosapentaenoic acid (EPA, 3-10 µM) reduces the contraction rate of cells, indicating a positive antiarrhythmic effect. Cytochrome P450 metabolites of EPA are more potent, with 17,18-epoxyeicosatetraenoic acid (17,18-EET) reducing contractions at 30 nM. CAY10665 is a bioisostere of 17,18-EET which is approximately 50% more effective at reducing arrhythmic contraction frequency, without affecting amplitude, when tested at 30 nM. This disubstituted oxamide represents a stable analog of 17,18-EET which may be used to study the mechanism of regulation of cardiomyocyte contractility by EPA metabolites.Formal Name: 16-[[2-(methylamino)-2-oxoacetyl]amino]-11Z-hexadecenoic acid. CAS Number: 1235543-17-5. Molecular Formula: C19H34N2O4. Formula Weight: 354.5. Purity: >98%. Formulation: (Request formulation change), A solution in ethanol. Solubility: DMF: 30 mg/ml, DMSO: 30 mg/ml, Ethanol: 30 mg/ml. SMILES: O=C(C(NC)=O)NCCCC/C=C\CCCCCCCCCC(O)=O. InChi Code: InChI=1S/C19H34N2O4/c1-20-18(24)19(25)21-16-14-12-10-8-6-4-2-3-5-7-9-11-13-15-17(22)23/h6,8H,2-5,7,9-16H2,1H3,(H,20,24)(H,21,25)(H,22,23)/b8-6-. InChi Key: WKESFWWKRDQART-VURMDHGXSA-N.